Affinity purified Lotus tetragonolobus lectin (LTL) is isolated from lotus seeds and is composed of 240 amino acid residues. LTL is a glycoprotein consists of four subunits. This lectin has different isoelectric points of pH 7.3, 7.6, 7.9, 8.2 and a carbohydrate specificity toward α-fucose, it is most inhibited by L-fucose. LTL is a fucose-binding anti-H(O) lectin. Transitional cell carcinoma of the human urinary bladder tends to metastasize when it strongly expresses binding sites for LTL.
Colloidal gold can be used to visualize localization of specific saccharide moieties and examine the distribution of plasma membrane glycoproteins based on the binding pattern of Lotus tetragonolobus Lectin (LTL). Colloidal gold labeled lectins can be viewed via electron microscopy.