Lens culinaris Lectin (LCA/LCH) - Colloidal Gold

Lens culinaris Lectin (LCA/LCH) - Colloidal Gold
Lens culinaris (Lentil) Lectin (LCA/LCH) - Colloidal Gold
Product image 1Lens culinaris Lectin (LCA/LCH) - Colloidal Gold
Product image 2Lens culinaris (Lentil) Lectin (LCA/LCH) - Colloidal Gold

Price: Regular price $144.98

SKU#: 21510828-1

Product Description

Affinity purified Lens culinaris lectin (LCA) is composed of four subunits that consists of an α-chain (MW: 5.7 kDa) and β-chain (MW: 17.5 kDa). It has a carbohydrate specificity toward α-D-mannose and α-D-glucose, eluting with the sugar MeαMan+MeαGlc. This lectin is useful in affinity chromatography columns for the separation of glycoconjugates. The two isomers LCA-A and LCA-B agglutinates human red blood cells, although not blood group specific. Activity of LCA is determined by haemagglutination with human blood and agglutinates a 2% suspension of human erythrocytes at a lectin concentration of 8µg/ml. LCA requires calcium and manganese ions for binding. See other LCA conjugates.

Colloidal gold can be used to visualize localization of specific saccharide moieties and examine the distribution of plasma membrane glycoproteins based on the binding pattern of Lens culinaris (Lentil) Lectin (LCA/LCH). Colloidal gold labeled lectins can be viewed via electron microscopy.

Technical Specifications

Abbreviation: LCA/LCH
Material Source:: Lentil
Conjugate:: Colloidal Gold
Concentration:: 1 mg/mL
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: α-Mannose, α-Glucose
Inhibiting or Eluting Sugar:: α-Methylmannoside + α-Methylglucoside
Divalent Ions:: Ca++, Mn++
Excitation:: 550 nm
Emission:: 570 nm
Mitogenic Activity:: Yes
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

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References

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