bioWORLDâ€šÃ Ã¶â€šÃ¢â€ s Intenseâ„¢ Protein Labeling Kit allows coupling of fluorescein to antibodies, lectins or any other proteins in a simple one step process. The Intenseâ„¢ Protein Labeling Kit is ideally suited to label antibodies, peptides, hormones, or other proteins as well as any other macromolecules that contain primary amino groups where fluorescein is attached. The labeling reagent reacts with primary amines (internal lysine residues or terminal amino groups) of the protein/ peptide, forming a stable covalent bond. Any unreacted fluorochrome is easily removed using spin columns (provided). Such fluorophore labeled proteins can be used in a variety of applications, such as ELISA assays, immunohistology, and fluorescent cell sorting. The Intenseâ„¢ Protein Labeling Kit contains all the necessary components for six labeling reactions having up to 1mg of protein per reaction. Kit components include: Purified fluorochrome: fluorescein isothiocyanate Fluorochrome Solvent Reaction Buffer Quenching Buffer 6 x 1mL Pre-packed Spin Columns 6 x 2mL Collection Tubes 6 Amber Tubes for storage of labeled protein.