BioWorld‚àö‚â†s Intense™ fluorochrome-conjugated Streptavidin and Avidin are highly purified reagents with very low non-specific reactivities. Since these proteins have a high affinity forBiotin, their fluorescent labeled conjugates (developed for maximum fluorescence per unit of protein) can be used to detect macromolecules such as,Biotinylated secondary antibodies, lectins and other proteins for applications in immunofluorescence, in-situ hybridization, flow cytometry, and ELISA assays. Further amplification of fluorescent signals can be achieved withBiotinylated secondary antibodies followed by fluorochrome-labeled Streptavidin or Avidin reagents. Thus, aBiotin: avidin orBiotin: streptavidin detection system provides significant amplification of a signal in comparison to a directly conjugated secondary antibody used for the detection of a target. Intense™ Fluorescent Streptavidin Kit contains 250 µg of three fluorescently labeled Streptavidin conjugates:AMCA Streptavidin,Texas Red‚àö√ú Streptavidn, and Fluorescein Streptavidin. Fluorescein Streptavidin is a green-yellow fluorescence that excites at 495 nm and emits at 515 nm.Texas Red‚àö√ú Streptavidin is deep red and excites at 595 nm and emits at 615 nm.AMCA- Streptavidin is blue and excites at 350 nm and emits at 450 nm, providing the option of introducing multiple labels into a section. This panel of fluorescent streptavidin products provides sufficient reagent to stain 200 ‚àö¬± 400 frozen or paraffin embedded sections.