Affinity purified Hippeastrum hybrid lectin (HHL) is consists of four subunits of identical size. It binds only to (α-1,3) and (α-1,6) linked mannosyl units of α-mannose residues. HHL reacts not only with terminal but also with internal α-D-mannosyl residues. Lectin appears to possess extended binding sites complementary to at least three 1,6-linked α-mannosyl. HHL also binds to some yeast galactomannans.
Colloidal gold can be used to visualize localization of specific saccharide moieties and examine the distribution of plasma membrane glycoproteins based on the binding pattern of Hippeastrum hybrid Lectin (HHL). Colloidal gold labeled lectins can be viewed via electron microscopy.