DAB (3,3'-diaminobenzidine), anHRP substrate, produces dark brown reaction product and is used for both immunohistochemical and Western blotting applications. The bioWORLDâ€šÃ Ã¶â€šÃ¢â€ s Formaxâ„¢ DAB substrate is a purified chromogen which is effective as a single stain or as a second color for double labeling and provide 2-4 fold greater sensitivity than original Substrate Kit. Since the DAB reaction product is heat-resistant, it can be used in both IHC and ISH labeling applications. Sections developed with DAB can be dehydrated, cleared, and permanently mounted in organic media. Imaging software systems can distinguish DAB from otherHRP or AP enzyme substrates where multiple antigens are co-localized. This substrate can also be used for electron microscopy. The DAB reaction product can be intensified with DAB Enhancing Solution (see SKU 21760044-1). See also Intenseâ„¢ DAB (SKU 21760043-1) which is 3-4 times more sensitive. Suitable for IHC/ICC/ISH and Western blot analysis.