BCIP-NBTAlkaline Phosphatase (AP) Substrate forms an indigo, heat stable reaction product. Sections treated with this substrate can be dehydrated, cleared, and permanently mounted. This chromogen can be used singly or in combination with otherAlkaline Phosphatase or peroxidase substrates for multiple labeling applications. This substrate can also be used for developing Western blots or for in situ hybridization studies. The sensitivity of this substrate is equivalent to the otherAlkaline Phosphatase substrates taking only 20-30 minutes development time. Since BCIP-NBT reaction continues to develop for several hours, it makes it one of the most sensitive chromogenic substrates for AP. ThisKit contains stock solutions in convenient ReadyGoâ„¢ dropper bottles, saving time for processing samples in immunohistology applications.