The ForMax™ Blue-APAlkaline Phosphatase Substrate Kit produces an intense blue color when reacted byAlkaline Phosphatase. This chromogen is heat stable and can be used in combination with otherAlkaline Phosphatase or peroxidase substrates for multiple labeling applications. The spectral profile of this substrate can be distinguished from other enzyme substrates using imaging systems and software in applications where antigens are co-localized. Sections stained with the ForMax™ Blue substrate can be dehydrated, cleared, and permanently mounted using organic media. The reaction product is soluble in xylene, but limonine can be used.