Euonymus europaeus Lectin (EEL/EEA) - Pure

Euonymus europaeus Lectin (EEL/EEA) - Pure

Price: Regular price $96.45

SKU#: 21510004-1

Product Description

Euonymus europaeus lectin or agglutinin (EEL/EEA) is affinity purified and is a homodimer consisting of subunits of 17 kDa linked by disulfide bonds. EEL consists of six closely related lectins (isoforms). Previous reports of higher molecular weight and overestimated carbohydrate percentage are attributed to lectin-glycoprotein complexes and not the Euonymus europaeus lectin itself. This lectin is characteristic of its erythrocyte agglutinating properties and specificity for blood groups B and H. It has a stronger binding of the B oligosaccharide with higher affinity for B-type II in comparison to H structures with Galα1,3 structures. Conversely, it has also been reported that EEL has preferential binding to H blood group-related carbohydrates over the B bloof group due to similarities in the β-trefoil fold found in EEL and ricin B-like lectins. EEL’s general pattern allows it to interact with both α-fucose residues on B and H blood groups as well as high mannose N-type glycans. This lectin binds to endothelial cells from human and non-human sources and recognizes carbohydrate structures on the surface of stimulated murine peritoneal lymphoid cells. EEL also has applications as a endothelial/epithelial marker in canine tissues and has been studied in Kashin-Beck disease. See other EEL/EEA conjugates.

 

 

Specifications:

  • Source: Euonymus europaeus (Spindle tree)
  • Carbohydrate Specificity: Galα3Gal
  • Activity: 50 mg/ml will agglutinate human A2 erythrocytes
  • Inhibitory Carbohydrate: Lactose
  • Divalent Ions Required: Ca++, Zn++

 

Storage and Stability:

Store frozen at -20°C in amber vials in appropriate aliquot sizes. Avoid freeze thaw cycles. Can be stored at 2-8°C for short term use.

 

Technical Specifications

Abbreviation: EEL/EEA
Material Source:: Spindle tree
Conjugate:: None
Molecular Weight:: 140 kDa
Purity:: High Purity Grade
Shelf Life:: 2 years
Blood Group Specificity:: O (-SA), B
Preferred Sugar Specificity:: Galα3Gal
Inhibiting or Eluting Sugar:: Lactose
Divalent Ions:: Ca++, Zn++
Mitogenic Activity:: Yes
Lyophilized or Liquid: Lyophilized
Storage Temperature:: -20°C
Hazardous Shipping:: Non-hazardous

Applications

Glycobiology, Agglutination studies, Endothelial cell-binding

References

Close (esc)

10% Discount!

Sign up for our mailing list below and you will receive 10% off your first order of $500.00 or more.

Age verification

By clicking enter you are verifying that you are old enough to consume alcohol.

Search

Shopping Cart

Your cart is currently empty.
Shop now