Euonymus europaeus Lectin (EEL/EEA) - Biotinylated

Euonymus europaeus Lectin (EEL/EEA) - Biotinylated

Price: Regular price $159.30

SKU#: 21510054-1

Product Description

Euonymus europaeus lectin or agglutinin (EEL/EEA) is affinity purified and is a homodimer consisting of subunits of 17 kDa linked by disulfide bonds. EEL consists of six closely related lectins (isoforms). Previous reports of higher molecular weight and overestimated carbohydrate percentage are attributed to lectin-glycoprotein complexes and not the Euonymus europaeus lectin itself. This lectin is characteristic of its erythrocyte agglutinating properties and specificity for blood groups B and H. It has a stronger binding of the B oligosaccharide with higher affinity for B-type II in comparison to H structures with Galα1,3 structures. Conversely, it has also been reported that EEL has preferential binding to H blood group-related carbohydrates over the B bloof group due to similarities in the β-trefoil fold found in EEL and ricin B-like lectins. EEL’s general pattern allows it to interact with both α-fucose residues on B and H blood groups as well as high mannose N-type glycans. This lectin binds to endothelial cells from human and non-human sources and recognizes carbohydrate structures on the surface of stimulated murine peritoneal lymphoid cells. EEL also has applications as a endothelial/epithelial marker in canine tissues and has been studied in Kashin-Beck disease. Biotin is a small molecule involved in a wide range of metabolic processes. This ligand forms a complex with Avidin and Streptavidin, resulting in the strongest non-covalent protein-ligand interaction known. Biotinylated EEL has an appropriate amount of biotin bound to provide optimum detection characteristics when using an Avidin-HRP or Streptavidin-HRP conjugate. Biotinylated lectins allow for more sensitive detection in ELISA and Western-blotting applications.

 

Specifications:

  • Source Euonymus europaeus (Spindle tree)
  • Activity: 50 mg/ml will agglutinate human A2 erythrocytes.
  • Carbohydrate Specificity: Galα3Gal, high mannose N-type glycans
  • Inhibitory Carbohydrate: Lactose
  • Divalent Ions Required: Ca++, Zn++
  • Conjugation: Biotin 
Storage and Stability:
Store frozen at -20°C in amber vials or covered with foil in appropriate aliquot sizes. Avoid freeze thaw cycles. Can be stored at 2-8 °C for short term use.  Clarify by centrifugation, if needed.

Technical Specifications

Abbreviation: EEL/EEA
Material Source:: Spindle tree
Conjugate:: Biotinyl N- Hydroxysuccinimide Ester (Biotin)
Concentration:: 2 mg/mL
Molecular Weight:: 140 kDa
Appearance Form:: Liquid
Appearance Color:: Clear, Colorless
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: Galα3Gal
Inhibiting or Eluting Sugar:: Lactose
Divalent Ions:: Ca++, Zn++
Mitogenic Activity:: Yes
Lyophilized or Liquid: Liquid
Storage Temperature:: -20°C
Hazardous Shipping:: Non-hazardous

Applications

References

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