Erythrina cristagalli lectin or agglutinin (ECL/ECA) is affinity purified lectin consists of two subunits with molecular weights of 28,000 and 26,000. ECL binds to carbohydrate structure in membrane and serum glycoproteins of mammalian cells. The shape of the lectin binding domains may resemble cavity type with GalÎ²1->4GlcNAc as the core binding site with additional one to four sugar subsites and is most complementary to a linear trisaccharide. Some GalÎ²1-related oligosaccharides are the major structures for lectin binding. Lectin binding activity can be abolition through salic acid substitution. This allows for specificity to fractionate and isolate mammalian glycoproteins. MicroPass™ microcolumns are prepared by conjugating Separopore® 4B with affinity purified (AffyPure™) Erythrina cristagalli (Coral tree) Lectin (ECL/ECA). These microcolumns are used for MS and proteomic analysis to analyze of <100µL samples.