Dolichos biflorus Lectin (DBA) - Colloidal Gold

Dolichos biflorus Lectin (DBA) - Colloidal Gold
Dolichos biflorus (Horse gram) Lectin (DBA) - Colloidal Gold
Product image 1Dolichos biflorus Lectin (DBA) - Colloidal Gold
Product image 2Dolichos biflorus (Horse gram) Lectin (DBA) - Colloidal Gold

Price: Regular price $186.70

SKU#: 21510816-1

Product Description

Dolichos biflorus agglutinin (DBA) is isolated from horse gram and purified by affinity chromatography. DBA is a glycoprotein with a molecular weight of about 111,000 and consists of 4 subunits of equal size. This lectin has an isoelectric point between pH 4.6 and pH 5.5. It has a carbohydrate specificity toward α-N-acetylgalactosamine and elutes with the sugar GalNAc.

DBA has been used as a general marker of developing renal collecting ducts, which can then be used to establish secretion status in blood group A individuals by hemagglutination inhibition and for detecting blood typing. DBA is over thousand times more active in agglutinating blood group A1 cells versus A2 cells, and is used to distinguish these blood types in routine serology applications, and has potential applications in examining tumors which affect the levels of blood group substances.

Reconstitute with sterile buffer. Calcium, manganese, magnesium, and zinc ions are required for binding.

Colloidal gold can be used to visualize localization of specific saccharide moieties and examine the distribution of plasma membrane glycoproteins based on the binding pattern of Dolichos biflorus Lectin (DBA). Colloidal gold labeled lectins can be viewed via electron microscopy.

Technical Specifications

Abbreviation: DBA
Material Source:: Horse gram
Conjugate:: Colloidal Gold
Concentration:: 1 mg/mL
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: α-N-Acetylgalactosamine
Inhibiting or Eluting Sugar:: N-Acetylgalactosamine
Mitogenic Activity:: No
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

Applications

References

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