{"product_id":"concanavalin-a-lectin-con-a-dylight-488","title":"Concanavalin A Lectin (Con A) - DyLight 488","description":"\u003cp class=\"MsoNormal\" style=\"text-align: justify;\"\u003e\u003cspan style=\"color: black; mso-themecolor: text1;\"\u003eConcanavalin A Lectin (Con A) is isolated from jack bean seeds (Canavalia ensiformis) and purified by affinity chromatography. Con A is composed of identical subunits of 237 amino acid residues (MW: 26,000 without any cystine residues) and while above pH 7 it is predominantly tetrameric, at pH 4.5 - 5.6, Con A exists as a single dimer MW: 53,000. Con A binds with non-reducing α-D-glucose, α-D-mannose and α-methyl-D-glucopyranoside acts as a competitive inhibitor. It has a carbohydrate specificity towards α-mannose and α-glucose and elutes with MeαMan + MeαGlc. Con A does not have a blood group specificity. It exhibits mitogenic activity with lymphocytes and cancer cells which aggregate by Con A; normal white cells do not. This product comes in a stable lyophilized form\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 12.0pt; line-height: 115%; font-family: 'Aptos',sans-serif; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: Aptos; mso-fareast-theme-font: minor-latin; mso-hansi-theme-font: minor-latin; mso-bidi-font-family: 'Times New Roman'; mso-bidi-theme-font: minor-bidi; color: black; mso-themecolor: text1; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;\"\u003eDyLight 488 can be used to visualize the binding pattern of Concanavalin A (Jackbean) lectin (Con A) in cellular imaging and flow cytometry. DyLight 488 is a green-fluorescent dye that functions effectively over a broad pH range (pH 4-9). It is brighter and more photostable than many other green-fluorescent dyes, including FITC.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" style=\"text-align: justify;\"\u003e\u003cspan style=\"color: black; mso-themecolor: text1;\"\u003eRecommended usage: Recommended dilutions of 0.5 – 10 μg\/ml in 20 mM Tris-HCl pH 7.5, 50 mM NaCl, 1 mM CaCl2, 0.5 mM MnCl2. More than 15 μg\/ml can agglutinate rabbit\/human\/porcine erythrocytes.\u003c\/span\u003e\u003c\/p\u003e","brand":"GlycoMatrix™","offers":[{"title":"Default Title","offer_id":42568653930567,"sku":null,"price":0.0,"currency_code":"USD","in_stock":false}],"url":"https:\/\/www.glycomatrix.com\/products\/concanavalin-a-lectin-con-a-dylight-488","provider":"GlycoMatrix™","version":"1.0","type":"link"}