{"product_id":"concanavalin-a-lectin-con-a-alexa-fluor-488","title":"Concanavalin A Lectin (Con A) - Alexa Fluor 488","description":"\u003cp class=\"MsoNormal\" style=\"text-align: justify;\"\u003e\u003cspan style=\"color: black; mso-themecolor: text1;\"\u003eConcanavalin A Lectin (Con A) is isolated from jack beans (Canavalia ensiformis) and purified by affinity chromatography. Con A is composed of identical subunits of 237 amino acid residues (MW: 26,000 without any cystine residues) and while above pH 7 it is predominantly tetrameric, at pH 4.5 - 5.6, Con A exists as a single dimer MW: 53,000. Con A binds with non-reducing α-D-glucose, α-D-mannose and α-methyl-D-glucopyranoside acts as a competitive inhibitor. It has a carbohydrate specificity towards α-mannose and α-glucose and elutes with MeαMan + MeαGlc. Con A does not have a blood group specificity. It exhibits mitogenic activity with lymphocytes and cancer cells which aggregate by Con A (normal white cells do not). Normal cells react to Con A after proteolytic treatment which suggests that trypsinization causes clustering of the reactive glycan residues on the membrane. This product comes in a stable lyophilized form.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" style=\"text-align: justify;\"\u003e\u003cspan style=\"color: black; mso-themecolor: text1;\"\u003eConcanavalin A Lectin (Con A) conjugated with Alexa Fluor™ 488 is a bright, green, fluorescent dye that provides stable signal generation for imaging and flow cytometry. This conjugate is water soluble and pH-insensitive from pH 4 to pH 10, ensuring optimal performance across a range of experimental conditions. Alexa Fluor™ 488 enhances cellular labeling and detection capabilities, making it an ideal choice for sensitive applications in research.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" style=\"text-align: justify;\"\u003e\u003cspan style=\"color: black; mso-themecolor: text1;\"\u003eApplications: Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Elispot, Dot blot, flowcytometry, Cytotoxicity, Glycan Array, Glycobiology.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" style=\"text-align: justify;\"\u003e\u003cspan style=\"color: black; mso-themecolor: text1;\"\u003eRecommended Usage: Recommended dilutions of 0.5 – 10 µg \/ ml in 1XPBS. \u003c\/span\u003e\u003cspan style=\"font-family: 'Calibri',sans-serif; mso-fareast-font-family: Cambria; mso-bidi-font-family: 'Times New Roman'; color: black; mso-themecolor: text1;\"\u003eMore than 10 µg\/mL may be required to give visible agglutination of neuraminidase treated human erythrocytes.\u003c\/span\u003e\u003cspan style=\"color: black; mso-themecolor: text1;\"\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"GlycoMatrix™","offers":[{"title":"Default Title","offer_id":42206314627143,"sku":null,"price":163.53,"currency_code":"USD","in_stock":false}],"url":"https:\/\/www.glycomatrix.com\/products\/concanavalin-a-lectin-con-a-alexa-fluor-488","provider":"GlycoMatrix™","version":"1.0","type":"link"}