Cicer arietinum Lectin (CAL/CPA) - Separopore® 4B OnePASS™ Column

Product Description

Cicer arietinum lectin or agglutinin (CAL/CPA) is a homodimer composed of two subunits.This lectin is a glycoprotein that contains 4.5% neutral sugars and is basic in nature (pI: 9.0). It’s haemagglutination activity is inhibited by fetuin and desialylated fetuin with a carbohydrate specificity for fetuin and p-Nitrophenyl N-acetyl-α-D-galactoside. CAL possess complex-sugar specificity for N-glycans and N-acetyl-D-galactosamine, and elutes with acetyl-D-galactosamine. Although neither human or rabbit erythrocytes are agglutinated by this lectin, pronase-treated rabbit and human erythrocytes have been successfully agglutinated. None of the sugars, such as glucose, mannose or galactose, nor their derivatives, disaccharides, trisaccharides and tetrasaccharides have any effect on the agglutination of this lectin. Cicer arietinum Lectin (CAL/CPA) OnePASS™ Spin Column are standard Cicer arietinum Lectin (CAL/CPA) matrix prepacked in a column which can be used via gravity flow or as a spin column to rapidly separate bound and passthrough materials.