Cicer arietinum Lectin (CAL/CPA) - Cy3

Cicer arietinum Lectin (CAL/CPA) - Cy3
Cicer arietinum (Chickpea) Lectin (CAL/CPA) - Cy3
Product image 1Cicer arietinum Lectin (CAL/CPA) - Cy3
Product image 2Cicer arietinum (Chickpea) Lectin (CAL/CPA) - Cy3

Price: Regular price $141.85

SKU#: 21761163-1

Product Description

Cicer arietinum lectin or agglutinin (CAL/CPA) is a homodimer composed of two subunits.This lectin is a glycoprotein that contains 4.5% neutral sugars and is basic in nature (pI: 9.0). It’s haemagglutination activity is inhibited by fetuin and desialylated fetuin with a carbohydrate specificity for fetuin and p-Nitrophenyl N-acetyl-α-D-galactoside. CAL possess complex-sugar specificity for N-glycans and N-acetyl-D-galactosamine, and elutes with acetyl-D-galactosamine. Although neither human or rabbit erythrocytes are agglutinated by this lectin, pronase-treated rabbit and human erythrocytes have been successfully agglutinated. None of the sugars, such as glucose, mannose or galactose, nor their derivatives, disaccharides, trisaccharides and tetrasaccharides have any effect on the agglutination of this lectin.

Cy3 can be used to viualize the binding pattern of Cicer arietinum Lectin (CAL/CPA) in cellular imaging and flow cytometry. Cy3 is more photostable than many other flourophores and can be seen with TRITC filter sets. It is commonly combined with green-fluorescent dyes for dual-labeling.

Technical Specifications

Abbreviation: CAL/CPA
Material Source:: Chickpea
Conjugate:: Cyanine 3 (Cy3)
Concentration:: 1 mg/mL
Appearance Form:: Liquid
Appearance Color:: Orange
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: Complex N-Glycans
Inhibiting or Eluting Sugar:: Human IgM, Bovine Fetuin
Fluorescence:: Orange
Divalent Ions:: Ca++, Mn++
Excitation:: 550 nm
Emission:: 570 nm
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

Applications

References

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