Cicer arietinum Lectin (CAL/CPA) - Cy3
Price: Regular price $141.85
Product Description
Cicer arietinum lectin or agglutinin (CAL/CPA) is a homodimer composed of two subunits.This lectin is a glycoprotein that contains 4.5% neutral sugars and is basic in nature (pI: 9.0). It’s haemagglutination activity is inhibited by fetuin and desialylated fetuin with a carbohydrate specificity for fetuin and p-Nitrophenyl N-acetyl-α-D-galactoside. CAL possess complex-sugar specificity for N-glycans and N-acetyl-D-galactosamine, and elutes with acetyl-D-galactosamine. Although neither human or rabbit erythrocytes are agglutinated by this lectin, pronase-treated rabbit and human erythrocytes have been successfully agglutinated. None of the sugars, such as glucose, mannose or galactose, nor their derivatives, disaccharides, trisaccharides and tetrasaccharides have any effect on the agglutination of this lectin.
Cy3 can be used to viualize the binding pattern of Cicer arietinum Lectin (CAL/CPA) in cellular imaging and flow cytometry. Cy3 is more photostable than many other flourophores and can be seen with TRITC filter sets. It is commonly combined with green-fluorescent dyes for dual-labeling.
Technical Specifications
| Abbreviation | : | CAL/CPA |
| Material Source: | : | Chickpea |
| Conjugate: | : | Cyanine 3 (Cy3) |
| Concentration: | : | 1 mg/mL |
| Appearance Form: | : | Liquid |
| Appearance Color: | : | Orange |
| Shelf Life: | : | 1 year |
| Blood Group Specificity: | : | Non-specific |
| Preferred Sugar Specificity: | : | Complex N-Glycans |
| Inhibiting or Eluting Sugar: | : | Human IgM, Bovine Fetuin |
| Fluorescence: | : | Orange |
| Divalent Ions: | : | Ca++, Mn++ |
| Excitation: | : | 550 nm |
| Emission: | : | 570 nm |
| Lyophilized or Liquid | : | Liquid |
| Storage Temperature: | : | 2-8°C |
| Hazardous Shipping: | : | Non-hazardous |
