Cicer arietinum Lectin (CAL/CPA) - Colloidal Gold

Cicer arietinum Lectin (CAL/CPA) - Colloidal Gold
Cicer arietinum (Chickpea) Lectin (CAL/CPA) - Colloidal Gold
Product image 1Cicer arietinum Lectin (CAL/CPA) - Colloidal Gold
Product image 2Cicer arietinum (Chickpea) Lectin (CAL/CPA) - Colloidal Gold

Price: Regular price $186.70

SKU#: 21510810-1

Product Description

Cicer arietinum lectin or agglutinin (CAL/CPA) is a homodimer composed of two subunits.This lectin is a glycoprotein that contains 4.5% neutral sugars and is basic in nature (pI: 9.0). It’s haemagglutination activity is inhibited by fetuin and desialylated fetuin with a carbohydrate specificity for fetuin and p-Nitrophenyl N-acetyl-α-D-galactoside. CAL possess complex-sugar specificity for N-glycans and N-acetyl-D-galactosamine, and elutes with acetyl-D-galactosamine. Although neither human or rabbit erythrocytes are agglutinated by this lectin, pronase-treated rabbit and human erythrocytes have been successfully agglutinated. None of the sugars, such as glucose, mannose or galactose, nor their derivatives, disaccharides, trisaccharides and tetrasaccharides have any effect on the agglutination of this lectin.

Colloidal gold can be used to visualize localization of specific saccharide moieties and examine the distribution of plasma membrane glycoproteins based on the binding pattern of Cicer arietinum Lectin (CAL/CPA). Colloidal gold labeled lectins can be viewed via electron microscopy.

Technical Specifications

Abbreviation: CAL/CPA
Material Source:: Chickpea
Conjugate:: Colloidal Gold
Concentration:: 1 mg/mL
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: Complex N-Glycans
Inhibiting or Eluting Sugar:: Human IgM, Bovine Fetuin
Divalent Ions:: Ca++, Mn++
Excitation:: 550 nm
Emission:: 570 nm
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

Applications

References

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