Bauhinia purpurea Lectin (BPL/BPA) - Colloidal Gold

Bauhinia purpurea Lectin (BPL/BPA) - Colloidal Gold
Bauhinia purpurea (Camel's foot tree) Lectin (BPL/BPA) - Colloidal Gold
Product image 1Bauhinia purpurea Lectin (BPL/BPA) - Colloidal Gold
Product image 2Bauhinia purpurea (Camel's foot tree) Lectin (BPL/BPA) - Colloidal Gold

Price: Regular price $186.70

SKU#: 21510808-1

Product Description

Bauhinia purpurea agglutinin or lectin (BPA/BPL) is a tetrameric lectin with a molecular weight of 195,000. Binding appears to be highest for glycoconjugates containing galactosyl (β-1,3)N-acetylgalactosamine structures but oligosaccharides with a terminal α-linked N-acetylgalactosamine can also bind. BPA is lactose-specific and elutes with the sugar lactose. It has specificity for blood groups A, B, O (-SA). Treatment of erythrocytes with neuraminidase or trypsin will increase the agglutination reaction, indicating that the receptor is masked by terminal carbohydrates.

Although binding specificity is similar to that of peanut agglutinin, tissue staining patterns of these two lectins are distinct. Makela's group 2 sugars, particularly N-acetyl-D-galactosamine, are potent inhibitors. The native protein appears to be stable in detergent solution.

Colloidal gold can be used to visualize localization of specific saccharide moieties and examine the distribution of plasma membrane glycoproteins based on the binding pattern of Bauhinia purpurea Lectin (BPL/BPA). Colloidal gold labeled lectins can be viewed via electron microscopy.

Technical Specifications

Abbreviation: BPL/BPA
Material Source:: Camel's foot tree
Conjugate:: Colloidal Gold
Concentration:: 1 mg/mL
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: Galβ3GalNAc
Inhibiting or Eluting Sugar:: Lactose
Divalent Ions:: None Required
Excitation:: 550 nm
Emission:: 570 nm
Mitogenic Activity:: Yes
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

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